High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance
نویسندگان
چکیده
منابع مشابه
Full-field supercritical angle fluorescence microscopy for live cell imaging.
We introduce a full-field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering. This technique is based on supercritical emission selectivity. It can be implemented on any epifluorescence microscope with a commercial high numerical aperture...
متن کاملVirtual Supercritical Angle Fluorescence Microscopy for Real Time Membrane Imaging
We present an alternative full field imaging technique based on Supercritical Angle Fluorescence (SAF). When fluorescent emitters are placed in the vicinity of the glass slide, their near-field components become propagative at supercritical angles. This supercritical emission decays sharply the fluorophore/surface distance d with a characteristic decay length of about 100 nm. Selecting the supe...
متن کامل3D superresolution microscopy by supercritical angle detection.
We present a fundamentally new approach to 3D superresolution microscopy based on the principle of surface-generated fluorescence. This near-field fluorescence is strongly dependent on the distance of fluorophores from the coverslip and can therefore be used to estimate their axial positions. We established a robust and simple implementation of supercritical angle fluorescence detection for sin...
متن کاملNanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy.
We report a noninvasive fluorescence microscopy method and demonstrate nanometer resolution along the optical axis. The technique is based on the influence of the microscope slide on the angular intensity distribution of fluorescence. Axial positions are determined by measuring the proportion of light emitted below the critical angle of total internal reflection, which behaves in a classical wa...
متن کاملFast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising si...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics Express
سال: 2013
ISSN: 1094-4087
DOI: 10.1364/oe.21.022070